Tissue culture of agarwood tree (
Aquilaria
malaccensis Lam) was done to produce a large number of
transplants in the laboratory. The compositions of medium culture are commonly
modified to obtain the best growth of explants to regenerate into plantlets. Our
study aimed to evaluate and compare different medium formulations supplemented
with BAP on agarwood shoot culture growth. In this study we used MS, NN, B5, AR
and WP medium containing 0 (control treatment), 0.25, 0.5, 1.0 and 2.0 mg/L
BAP. The shoot tips were cultured for 16 weeks in the treatment media. Height
of shoots, number of leaves, number of adventive shoots, and number of roots
was recorded every week to determine the growth. The results showed that after
16 weeks of culture, the best medium for shoot height was NN medium with BAP
fortification (3.54cm) for number of leaves and adventive shoots proliferation
was Gamborg B5 medium fortified with 0.5 mg/L BAP (21.63 leaves and 8,38
shoots), while NN medium without BAP was best for rooting.
Hapsari, B. W., Rudiyanto, Martin, A. F., &
Ermayanti, T. M. (2021). The effect of medium formulation and BAP concentration
of growth and development of Aquilaria malaccensis Lam. shoot in vitro. ISIBIO
2020. IOP Conf. Series: Earth and Environmental Science 762 (2021) 012033. DOI:
https://doi.org/10.1088/1755-1315/762/1/012033
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