The aim of this research was to
compare the reduction effect of sucrose or table sugar concentration on growth
characteristics of red ginger cultured in MS
liquid medium. Shoots of red ginger was cultured on MS liquid medium without
addition of plant growth regulators, supplemented with 5, 10, and 20 g/l of
sucrose or table sugar for 8 weeks. Resulted plantlets were acclimatized in a
greenhouse to investigate their growth and survival rate. Numbers of stomata,
chlorophyll concentration as well as cross section of leaves from plantlets
grown in vitro were compared to those of transplants grown in the greenhouse. The
results showed that the use of table sugar at concentration of 20 g/l gave the
best growth of red ginger. Meanwhile, the reduction of table sugar from 20 to
10 g/l reduced growth and survival rate of in vitro shoots as well as that of
transplants in the greenhouse. Only few shoots formed roots when they were
grown on the medium containing 5 g/l of table sugar, and transplants failed to
grow in the greenhouse. It found that the chlorophyll content of in vitro
plantlets was lower than those of transplants grown in the glasshouse. However,
the number of stomata of the in vitro plantlets was higher than that of
transplants grown in the glasshouse. There was no anatomical abnormalities
found on the cross section of leaves between in vitro plantlets and transplants
grown in the greenhouse. The replacement of sucrose with table sugar may reduce
the production cost of plantlets.
Hapsari, B. W., Ermayanti, T. M., Rantau, D. E., & Rudiyanto, R. (2011, June). Comparison of the Reduction Effect of Sucrose and Table Sugar Concentration on Growth Characteristics of Red Ginger (Zingiber officinale Rocs.) Cultured in Liquid Medium. In Annales Bogorienses (Vol. 15, No. 1, pp. 15-20).
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